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41 |
Three-Dimensional Folding and Functional Organization Principles of the Drosophila Genome
Very interesting paper, that looks at how the genome is physically organized. It even finds interchromosomal DNA-DNA interactions!
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36402 |
|
42 |
Calcitonin-like diuretic hormones in insects
Nice summary of DH31 in different insects
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3018 |
|
43 |
Reading: insect hormones
Hormones Controlling Homeostasis in Insects
D.A. Schooley
F.M. Horodyski
G.M. Coast
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2330 |
|
44 |
Neuroactive Insecticides: Targets, Selectivity, Resistance, and Secondary Effects
Nice review on insecticide action, and why they don't kill us...
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2781 |
|
45 |
Useful study deck on the urea cycle
I had to enlarge it a bit to see clearly. Very useful, especially for the metabolomics side of the group.
|
1886 |
|
48 |
An in vivo functional analysis system for renal gene discovery in Drosophila pericardial nephrocytes
J Am Soc Nephrol. 2013 Feb;24(2):191-7. doi: 10.1681/ASN.2012080769. Epub 2013 Jan 4.
An in vivo functional analysis system for renal gene discovery in Drosophila pericardial nephrocytes.
Zhang F, Zhao Y, Han Z.
Source
Department of Internal Medicine, Division of Molecular Medicine and Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Abstract
The difficulty in accessing mammalian nephrons in vivo hinders the study of podocyte biology. The Drosophila nephrocyte shares remarkable similarities to the glomerular podocyte, but the lack of a functional readout for nephrocytes makes it challenging to study this model of the podocyte, which could potentially harness the power of Drosophila genetics. Here, we present a functional analysis of nephrocytes and establish an in vivo system to screen for renal genes. We found that nephrocytes efficiently take up secreted fluorescent protein, and therefore, we generated a transgenic line carrying secreted fluorescent protein and combined it with a nephrocyte-specific driver for targeted gene knockdown, allowing the identification of genes required for nephrocyte function. To validate this system, we examined the effects of knocking down sns and duf, the Drosophila homologs of nephrin and Neph1, respectively, in pericardial nephrocytes. Knockdown of sns or duf completely abolished the accumulation of the fluorescent protein in pericardial nephrocytes. Examining the ultrastructure revealed that the formation of the nephrocyte diaphragm and lacunar structure, which is essential for protein uptake, requires sns. Our preliminary genetic screen also identified Mec2, which encodes the homolog of mammalian Podocin. Taken together, these data suggest that the Drosophila pericardial nephrocyte is a useful in vivo model to help identify genes involved in podocyte biology and facilitate the discovery of renal disease genes.
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5265 |
|
50 |
Mosquitoes in Naure!
Vosshall apply their Drosophila skills to mutant mosquito to identify odorant receptors that are blocked by DEET.
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6441 |
|
51 |
A CAPA receptor in a tick
Gen Comp Endocrinol. 2013 Sep 17. pii: S0016-6480(13)00365-1. doi: 10.1016/j.ygcen.2013.09.001. [Epub ahead of print]
Molecular and functional characterization of the first tick CAP2b (periviscerokinin) receptor from Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).
Yang Y, Bajracharya P, Castillo P, Nachman RJ, Pietrantonio PV.
Source
Department of Entomology, Texas A&M University, College Station, TX 77843, USA.
Abstract
The cDNA of the receptor for CAP2b/periviscerokinin (PVK) neuropeptides, designated Rhimi-CAP2b-R, was cloned from synganglia of tick Rhipicephalus (Boophilus) microplus. This receptor is the ortholog of the insect CAP2b/PVK receptor, as concluded from analyses of the predicted protein sequence, phylogenetics and functional expression. Expression analyses of synganglion, salivary gland, Malpighian tubule, and ovary revealed Rhimi-CAP2b-R transcripts. The expression in mammalian cells of the open reading frame of Rhimi-CAP2b-R cDNA fused with a hemagglutinin tag at the receptor N-terminus was confirmed by immunocytochemistry. In a calcium bioluminescence assay the recombinant receptor was activated by the tick Ixodes scapularis CAP2b/PVK and a PVK analog with EC50s of 64 nM and 249 nM, respectively. Tick pyrokinins were not active. This is the first report on the functional characterization of the CAP2b/PVK receptor from any tick species which will now permit the discovery of the physiological roles of these neuropeptides in ticks, as neurohormones, neuromodulators and/or neurotransmitters
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1195 |
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52 |
A Survey of 6,300 Genomic Fragments for cis-Regulatory Activity in the Imaginal Discs of Drosophila melanogaster
Nice paper showing people thinking big in Columbia U (in NYC).
There are University funds to support visits to Columbia to generate collaborations...
|
980 |
|
54 |
TED talk: Oxitec and genetically modified mosquitoes
Worth a listen, especially if you aren't a regular TED user.
|
779 |
|
55 |
A Drosophila Model of High Sugar Diet-Induced Cardiomyopathy
Nice paper from Ross Cagan's lab in PLoS genetics. Metabolic pathways, fluxes, etc.
|
11127 |
|
56 |
Systematic genetic interaction screens uncover cell polarity regulators and functional redundancy
excellent paper on gene interactions in c.elegans from the Ahringer group
|
1393 |
|
58 |
Read this if you use KK lines!
You have been warned.
|
1096 |
|
60 |
Use of the viral 2A peptide for bicistronic expression in transgenic mice.
Useful alternative to the IRES sequence, if you want to express two transgenes at the same time.
|
1738 |
