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Display # |
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21 |
Anoctamin/TMEM16 family members are Ca2+-activated Cl− channels
another candidate for tubule Cl conductance?
|
970 |
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22 |
SuperSAGE - a new way of transcriptomics
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index (barcode) sequences are employed to discriminate tags from different samples. Such barcodes allow researchers to analyze digital tags from transcriptomes of many samples in a single sequencing run by simply pooling the libraries. Here, we demonstrated that HT-SuperSAGE provided highly sensitive, reproducible and accurate digital gene expression data. By increasing throughput for analysis in HT-SuperSAGE, various applications are foreseen and several examples are provided in the present study, including analyses of laser-microdissected cells, biological replicates and tag extraction using different anchoring enzymes.
|
9897 |
|
23 |
Extending Biochemical Databases by Metabolomic Surveys
Very much along the lines of what we're trying to do:
Metabolomics can map the large metabolic diversity in species, organs, or cell types. In addition to gains in enzyme specificity, many enzymes have retained substrate and reaction promiscuity. Enzyme promiscuity and the large number of enzymes with unknown enzyme function may explain the presence of a plethora of unidentified compounds in metabolomic studies. Cataloguing the identity and differential abundance of all detectable metabolites in metabolomic repositories may detail which compounds and pathways contribute to vital biological functions. The current status in metabolic databases is reviewed concomitant with tools to map and visualize the metabolome.
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8579 |
|
24 |
Fluorescent biosensors in your skin!
I've been wanting to try this for years - now someone has...
|
1989 |
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25 |
Enteric Neurons and Systemic Signals Couple Nutritional and Reproductive Status with Intestinal Homeostasis
Intersting paper about the interplay of endocrine system and osmoregulation, but in a different direction from usual ion transport folk.
|
1080 |
|
26 |
Prandiology of Drosophila and the CAFE assay
Excellent paper from Benzer's group - though perhaps surprising to see it in PNAS. They dared -and succeeded- in measuring food uptake rates and giving controlled doses of e.g. paraquat.
|
1190 |
|
27 |
RNA mimics of green fluorescent protein
Green fluorescent protein (GFP) and its derivatives have transformed the use and analysis of proteins for diverse applications. Like proteins, RNA has complex roles in cellular function and is increasingly used for various applications, but a comparable approach for fluorescently tagging RNA is lacking. Here, we describe the generation of RNA aptamers that bind fluorophores resembling the fluorophore in GFP. These RNA-fluorophore complexes create a palette that spans the visible spectrum. An RNA-fluorophore complex, termed Spinach, resembles enhanced GFP and emits a green fluorescence comparable in brightness with fluorescent proteins. Spinach is markedly resistant to photobleaching, and Spinach fusion RNAs can be imaged in living cells. These RNA mimics of GFP provide an approach for genetic encoding of fluorescent RNAs.
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1082 |
|
28 |
GAL4 System in Drosophila: A Fly Geneticist’s Swiss Army Knife
Nice overview of GAL4 and related advances
|
1339 |
|
29 |
Refinement of Tools for Targeted Gene Expression in Drosophil
Valuable up-to-date survey of transgenic twiddly bits
|
696 |
|
30 |
Peptide antagonist of V-ATPase - idea for a transgenic?
The peptide is :
ascngvcspf emppcgtsac rcipvglvig ycrnpsg
- short enough to synthesise as oligos?
|
7430 |
|
31 |
V-ATPases, SNARES and membrane fusion
Interesting and potentially important for us - do SNARES mediate control of V-ATPase by taking head-group on an off?
|
639 |
|
32 |
GM sterile release mosquitoes in the wild!
|
2085 |
|
33 |
A new technique for making tissues transparent!
Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain
|
682 |
|
34 |
Power tools for gene expression and clonal analysis in Drosophila
Useful listing of GAL4, GAL80, phiC31, lexA, etc...
|
2009 |
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35 |
Experimental Evidence Supports a Sex-Specific Selective Sieve in Mitochondrial Genome Evolution
Mitochondria are maternally transmitted; hence, their genome can only make a direct and adaptive response to selection through females, whereas males represent an evolutionary dead end. In theory, this creates a sex-specific selective sieve, enabling deleterious mutations to accumulate in mitochondrial genomes if they exert male-specific effects. We tested this hypothesis, expressing five mitochondrial variants alongside a standard nuclear genome in Drosophila melanogaster, and found striking sexual asymmetry in patterns of nuclear gene expression. Mitochondrial polymorphism had few effects on nuclear gene expression in females but major effects in males, modifying nearly 10% of transcripts. These were mostly male-biased in expression, with enrichment hotspots in the testes and accessory glands. Our results suggest an evolutionary mechanism that results in mitochondrial genomes harboring male-specific mutation loads.
N&V at http://www.sciencemag.org/content/332/6031/798.full.pdf
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8357 |
|
36 |
Visualizing adenosine-to-inosine RNA editing in the Drosophila nervous system
Brilliant paper - actually based on stuff they've been publishing over a few years - that uses a split GFP that only gets spliced together in cells that have an RNA editing enzyme. So you get fruitflies with editing-active tissues in green.
|
1248 |
|
37 |
A novel smooth septate junction-associated membrane protein, Snakeskin, is required for intestinal barrier function in Drosophila
Septate junctions (SJs) are the membrane specializations observed between epithelial cells in invertebrates. SJs play a crucial role in epithelial barrier function by restricting free diffusion of solutes through the intercellular space. In arthropod species, two morphologically different types of SJs have been described: pleated septate junctions (pSJs) and smooth septate junctions (sSJs), which are specific to ectodermal and endodermal epithelia, respectively. In contrast to the recent understanding of pSJ-related proteins, the molecular constituents of sSJs are mostly unknown. Here we report a novel sSJ-specific membrane protein, designated ‘Snakeskin’ (Ssk). Ssk is highly concentrated in sSJs in the Drosophila midgut and Malpighian tubules. Lack of Ssk expression is embryonically lethal in Drosophila and results in defective sSJ formation accompanied by abnormal morphology of midgut epithelial cells. We also show that the barrier function of the midgut to a fluorescent tracer is impaired in Ssk-knockdown larvae. These results suggest that Ssk is required for the intestinal barrier function in Drosophila.
|
663 |
|
38 |
UK doubles the number of genetic diseases screened at birth
Useful for our IEM/metabolomics and human genetics interest groups...
|
2976 |
|
39 |
Systems genetics of complex traits in Drosophila melanogaster
This describes the use of QTL mapping in Drosophila
|
1017 |
|
40 |
Drosophila cancer models
Recent review by Ross Cagan
|
3144 |
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