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There are 4 bits: Transilluminator – camera – laptop - printer.
For ease of setup, the camera, printer and particularly laptop should be left running.
Simple use
Stroke the trackpad to check the laptop is running & wake it up. Assuming that it is, and that you see the remote capture application running, just set up your gel in the centre of the transilluminator and click the shutter ‘release’ button in the 'capture' window on the laptop. (As the camera insists on auto-focussing, I like to put the fluorescent ruler alongside the gel, to give it something to work with.) These pictures are effectively free, so you can have several tries.
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Written by Administrator
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Wednesday, 10 November 2010 15:15 |
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Last Updated on Wednesday, 02 November 2011 17:30 |
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Drosophila saline - Julian (1994) |
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Compound
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mM
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g/l
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mOsM
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Dissolve together in about 800 ml water while stirring:
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NaCl
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117.5
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6.86
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235
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KCl
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20
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1.49
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40
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CaCl2.2H2O
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2
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0.29
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6
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MgCl2.6H2O
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2
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0.41
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6
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Glucose
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20
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3.96
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20
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HEPES
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8.6
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2.05
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12
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Dissolve each of these separately in about 100 ml water, then add together to the big beaker while stirring:
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NaHCO3
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10.24
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0.86
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20
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NaH2PO4
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4.5
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0.70
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15
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Adjust pH to 6.8.
Filter sterilise and store in fridge.
(Total Osmolality 354)
If the saline throws a precipitate, discard the saline- you'll have lost all the Ca++ and Mg++ as (very) insoluble phosphates.
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Written by Administrator
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Wednesday, 10 November 2010 14:21 |
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Last Updated on Wednesday, 10 November 2010 17:09 |
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This protocol works on tubule wholemounts. It is based on:
Sulphonylurea sensitivity and enriched expression implicate inward rectifier K+ channels in Drosophila melanogaster renal function. Jennifer M. Lee, Adrian K. Allan, Shireen A. Davies, Julian A.T. Dow J.exp.Biol. 2005
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Written by Julian Dow
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Wednesday, 10 November 2010 13:47 |
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Last Updated on Wednesday, 10 November 2010 16:36 |
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Pablo's tubule wholemount ICC for ER proteins |
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Wholemount immunocytochemistry for RyR.
All the solutions are kept and used at 4oC. Incubations and washes are performed at 4oC with gentle agitation on a flat bed rotary shaker.
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Written by Administrator
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Wednesday, 10 November 2010 14:02 |
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Last Updated on Wednesday, 10 November 2010 17:23 |
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Page 4 of 4 |
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