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Drosophila saline - Julian (1994) PDF Print E-mail

Compound 

mM

g/l

mOsM

Dissolve together in about 800 ml water while stirring:

NaCl

117.5

6.86

235

KCl

20

1.49

40

CaCl2.2H2O

2

0.29

6

MgCl2.6H2O

2

0.41

6

Glucose

20

3.96

20

HEPES

8.6

2.05

12

Dissolve each of these separately in about 100 ml water, then add together to the big beaker while stirring:

NaHCO3

10.24

0.86

20

NaH2PO4

4.5

0.70

15

Adjust pH to 6.8.

Filter sterilise and store in fridge.

(Total Osmolality 354)
If the saline throws a precipitate, discard the saline- you'll have lost all the Ca++ and Mg++ as (very) insoluble phosphates.

 

Written by Administrator   
Wednesday, 10 November 2010 14:21
Last Updated on Wednesday, 10 November 2010 17:09
 
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