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Drosophila saline - Julian (1994) |
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Compound
|
mM
|
g/l
|
mOsM
|
|
Dissolve together in about 800 ml water while stirring:
|
|
NaCl
|
117.5
|
6.86
|
235
|
|
KCl
|
20
|
1.49
|
40
|
|
CaCl2.2H2O
|
2
|
0.29
|
6
|
|
MgCl2.6H2O
|
2
|
0.41
|
6
|
|
Glucose
|
20
|
3.96
|
20
|
|
HEPES
|
8.6
|
2.05
|
12
|
|
|
|
|
|
Dissolve each of these separately in about 100 ml water, then add together to the big beaker while stirring:
|
|
NaHCO3
|
10.24
|
0.86
|
20
|
|
NaH2PO4
|
4.5
|
0.70
|
15
|
Adjust pH to 6.8.
Filter sterilise and store in fridge.
(Total Osmolality 354)
If the saline throws a precipitate, discard the saline- you'll have lost all the Ca++ and Mg++ as (very) insoluble phosphates.
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Written by Administrator
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Wednesday, 10 November 2010 14:21 |
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Last Updated on Wednesday, 10 November 2010 17:09 |
Protocol section